Introduction to LC-MS for Pharma Industry

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The course will commence with registration and coffee at 8.30, course proper starts at 9.00 and will finish at 17.00

09:00 Introduction.

  • Identifying the pharmaceutical application areas for LC-MS.
  • Principles of LC-MS and LC-MS-MS.
  • Data acquisition strategies in LC-MS.

10:30 Coffee break

10:45 Solutions by LC-MS:

  • Instrumentation overview.
  • Electrospray and APCI.
  • Flow-rate and mobile-phase composition.
  • Different types of mass analysers: how and when about single quadrupole, triple quadrupole, ion-trap, and time-of-flight instruments.

12:30 Lunch


  • Potential and limitations of LC-MS in quantitative (bio)analysis.
  • General workflow in quantitative analysis.
  • Selected reaction monitoring.
  • Analytical strategies involved in bioavailability, PK/PD studies, metabolic stability, genotoxic impurities.
  • Validation issues.

15:15 Coffee break


  • LC-MS in qualitative applications.
  • Fragmentation in MS-MS as a tool for structure elucidation, Multistage MSn and accurate-mass determination.
  • Software tools.
  • Applications in checking compound identity, impurity profiling, and metabolite identification.

17:00 End of the course

Wilfried Niessen

Wilfried Niessen

Wilfried has almost 30 years of experience as a researcher and project manager in the field of analytical mass spectrometry, and especially liquid chromatography mass spectrometry. Since 1996, he has worked as an independent consultant, providing expert consultancy and (advanced) courses in analytical mass spectrometry. In 2002, he was appointed (part-time) extraordinary professor in bioanalytical mass spectrometry at the Faculty of Science of the VU University in Amsterdam, where he is currently interim Head of the Division BioMolecular Analysis.

Wilfried has been involved in many different consultancy projects within industry, governmental institutes and other laboratories in the Netherlands, Belgium, Italy, Germany, Denmark, Sweden, United Kingdom, Switzerland, Slovenia, Israel, Hong Kong China, India, and Canada. In addition, in the past 14 years he provided more than 320 courses with in total more than 3400 participants. He is (co)author of more than 160 refereed papers in the field of LC-MS. The third edition of his book Liquid Chromatography - Mass Spectrometry was published in October 2006. He co-edited several special volumes of Journal of Chromatography A (794, 970, 974, 1058, 1067, and 1159) on "Mass Spectrometry: Innovation and Application". He is the editor of Volume 8: Hyphenated Methods of the Encyclopaedia of Mass Spectrometry, published in 2006 by Elsevier.



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