- GMP and Compliance
- Pharmaceutical Product Development
- Development and Manufacture of Effervescent Tablets
- HPLC Analytical Method Development and Validation
- Hands-on Tablet Development including the principles of pre-formulation, formulation and process dev
- Introduction to LC-MS for Pharma Industry
- Introduction to Photostability
- Introduction to Sterile Product Manufacture for New Starters and Junior Staff
- Introduction to Sterile Product Manufacture for those with a cGMP basic understanding
- Pharmaceutical Packaging - an introductory course
- Pharmacokinetics in Drug Development - an integrated approach
- Preformulation Studies for Tablet Development
- QbD and Lifecycle Management for Analytical Methods
- Quantitative Bioanalysis using LC-MS for Pharma Industry
- Stability Testing in Biological Product Development and Manufacturing
- Stability Testing in Pharmaceutical Development and Manufacture
- Tablet Formulation Development
- Tablet Formulation Development - Formulation development in practice
- Tablet Process Development, Validation and the Application of QbD
- Technology Transfer
- Pharmaceutical Manufacturing Support
- Pharmaceutical R&D Support
- In-house Training Courses
Quantitative Bioanalysis using LC-MS for Pharma Industry
| Tutor : William van Dongen and Wilfried Niessen | |||
| Date | Location | Fee | |
|---|---|---|---|
| 25 & 26 September 2012 | London | £1,062 + Vat Book now for early bird price |  Before 23 July 2012 |
Overview
This advanced application-oriented two-day course in LC-MS covers the important aspects of quantitative bioanalysis by LC-MS (except for statistical aspects in data-processing). Starting with providing knowledge on important processes in analyte ionization by electrospray or APCI, the course provides a profound understanding of the important topics of quantitative bioanalysis using LC-MS. Quality control in instrument performance, method development, and systematic troubleshooting are key issues throughout the course.
The theoretical sections of the course are illustrated with a number of case studies.
Experience with the LC-MS instrumentation is recommended prior to attending this course.
The aim is to provide the participants with the general principles, background knowledge, and how to use this knowledge in practice.
Course Programme
The course will commence with registration and coffee at 8.30, course proper starts at 9.00 and will finish at 17.00
Day 1
09:00 Introduction. Introduction to LC-MS: System overview. Mass analyzers. Data-acquisition and processing. Role of LC-MS in quantitative bioanalysis.
10:30 Coffee break.
10:45 Analyte ionisation for LC-MS: electrospray and atmospheric-pressure chemical ionisation. Instrumentation, chemistry, ionisation mechanism, adduct ions, flow-rates, mobile-phase composition. Introduction to matrix effects.
12:30 Lunch break.
13:30 Method development 1: General workflow in quantitative analysis using LC-MS. MS aspects: Selected reaction monitoring (SRM). Selection of transitions. Selection of an appropriate internal standard.
15:00 Coffee break.
15:15 Method development 2: Method validation aspects: What are the rules? Why are the rules as they are? How to deal with them in practice? Consequences for method development.
17:00 End of the first day.
Day 2
09:00 Method development 3: Mass spectrometry aspects. How to appreciate the information that is present in the analyte mass spectrum, e.g., adduct ions and adduct bound dimers, but also topics related to isotope peaks. Multiresidue analysis and SRM. Ion-traps and high-resolution instruments in quantitative bioanalysis.
10:30 Coffee break.
10:45 Method development 4: Sample pre-treatment methods and liquid chromatography aspects for bioanalysis. Directives for method development.
12:30 Lunch.
13:50 Method development 5: Matrix effects. Methods to evaluate sample pre-treatment and separation methods with respect to matrix effects.
15:00 Coffee break.
15:15 Method development 6: Demonstrating the complete workflow with selected applications.
17:00 End of the second day and end of the course.
